Structural data of dna binding and molecular docking. Results indicate the dna binding affinities of complexes follow the order 3 4 1 2 5 6. Intercalation is the most possible mode of interaction of the ligand h 2 l and its mii complexes with ct. Binding studies of metalsalphen and metalbipyridine complexes. Thus through a series of experiments in this thesis, attempts have been made to correlate the structure and dynamics of metal complexes bound to dna. Dnabinding properties of these metal complexes were investigated using absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and thermal denaturation methods. The complexes have shown to interact strongly with quadruplex dna with binding constants of ca. Dna binding properties of these metal complexes were investigated using absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and thermal denaturation methods.
Doublehelical dna can bend,6 form loops and cruciforms,7 and fold. Establishing correlations among structure, dynamics and reactivity is a fundamental problem in biological chemistry. The schiff bases are an important class of ligands in coordination. Experimental studies suggest that the complexes bind to dna through intercalation. Synthesis, structural characterization, and quadruplex dna. Transition metal complexes have attracted curiosity due to dna binding and cleavage properties under physiological conditions. Dnabinding metallointercalators are positively charged, planar, polycyclic, aromatic compounds that unwind the dna double helix and insert themselves between dna base pairs. These include complexes of transition metals from groups 812 with dozens of combinations of ligands and oxidation states. Synthesis, characterization, dna binding properties. Synthesis, characterization, dna binding and cleavage studies.
First, the effects of intervening mismatches on dna structure, dynamics and dna charge transport reactivity is examined. From all the spectral data a distorted octahedral geometry has proposed for both the complexes. Design, syntheses and functional studies of dna binding. Mar 30, 2009 metal ions and metal complexes have long been recognized as critically important components of nucleic acid chemistry, both in regulation of gene expression and as promising therapeutic agents. Synthesis, characterization, biological activity and.
Synthesis, characterization and dna binding study of. Pdf metal complex interactions with dna researchgate. T1 characterization of the dna binding specificity of shelterin complexes. Dna cleaving abilities of many transition metal derivatives have been studiect. Here we describe recent results on a variety of transition metal complexes targeted to dna to illustrate. Molecular modelling studies were also performed to confirm the geometries of the complexes. An external file that holds a picture, illustration, etc. Nuclease digestion studies have suggested that hu binding alters the helical pitch 9, and circularization assays are indicative of dna bending upon hu binding 10, 11. Significant attention has centered upon metal complexes capable of binding dna by intercalation 36, and, in particular, due to their luminescent properties and. Dnacleaving abilities of many transition metal derivatives have been studiect. The results of these studies showed that dna binding strengthened as the size of the unique ligand was increased. Metal complexes interact with dna via covalent binding and most aromatic moieties bind dna via non. Materials and methods all the reagents and solvents used for the synthesis of ligand and the metal complexes were analar grade of.
Evaluation of dna binding and cytotoxic activity of a new. The dna binding capability of the four novel transition metal complexes containing macroacyclic schiff base ligand of the type n 1,n 8bis9benzyliminophenanthren10ylidenenaphthalene1,8. Synthesis, characterization and dna binding study of mixed. The ligand and their metal complexes were screened for their antimicrobial activity against bacteria. Sep 03, 2009 the complexes have shown to interact strongly with quadruplex dna with binding constants of ca. Synthesis, spectroscopic, thermal properties, calf thymus dna. An overview is presented of the key role for heavy metal compounds in medicine with a focus on dna binding, and on pt compounds used in treatment of cancer. Zn 3, and finally to the fully zincloaded active form zur 2. The effect of direct or indirect binding of intercalant molecules on dna structure is of fundamental importance in understanding the biological functioning of. Coordination compounds are formed between a metal atom or newlineion and a molecule.
Metal complexes can interact with nucleic acids through a variety of different modes, with the particular mode of binding being predictably governed by the metal complex structure. The focus of this work is introduction of gelred gr as a stable, sensitive and environmentally safe fluorescent dna dye instead of the highly toxic ethidium bromide eb. Uvvis spectroscopy studies of the metal complexes and precursor diyne show strong. In previous studies of hu binding to linear dna, multiple complexes were detected in. Read studies on dna binding behaviour of biologically active transition metal complexes of new tetradentate n 2 o 2 donor schiff bases. Interactions of metal complexes with dna jihan talib university of wollongong talib, jihan, interactions of metal complexes with dna, phd thesis, school of chemistry. Synthesis, characterization, antimicrobial, dna binding. The interactions between the metal complexes and dna were studied using electronic absorption method. Synthesis, characterization and dna binding study of ruthentumii thiosemicarbazone complexes k. The in silicon dna binding reveals copper complex is bound to be minor. Binding studies of 4, 5a, and 5b against calf thymus dna were carried out, revealing that 4, 5a, and 5b have. The absorption spectra of the niii complex in the absence and presence of ctdna are given in figure 2.
Synthesis, dna binding, and antimicrobial studies of novel. Dnaprotein binding, molecular docking, and in vitro. Analyses of metalbinding affinities reveals negative cooperativity between the two site 2 binding events in each dimer. Electronic absorption spectroscopy is universally employed to determine the binding characteristics of metal complexes with dna 3032. Jayabalakrishnan2 1department of chemistry, kumaraguru college of technology, coimbatore, tn, india 2department of chemistry, sri ramakrishna mission vidyalaya college of arts and science, coimbatore,tn, india.
Numerous biological experiments have also demonstrated that dna is the primary intracellular. N2 the shelterin complex associates with telomeres and plays an essential role in telomere protection and telomerase regulation. The dna cleavage studies of metal complexes presented more protruding. Binding of the complex to dna lead to perturbation in their ligand centered band. Intercalation processes of copper complexes in dna. Synthesis, characterization, antimicrobial, dna binding and. Transition metal complexes have attracted considerable attention as catalytic systems for use in the oxidation of organic compounds 2, probes in electrontransfer reactions involving metalloproteins 3.
Mar 30, 2010 in addition, dna binding properties of the ligand and its metal complexes have been investigated by electronic absorption spectroscopy, fluorescence spectra, ethidium bromide displacement experiments, iodide quenching experiments, salt effect and viscosity measurements. On the basis of a structural analysis of 240 proteindna complexes contained in the protein data bank pdb, we have classified the dnabinding proteins involved into eight different structuralfunctional groups, which are further classified into 54 structural families. Noncoordinative interactions with dna duplexes, quadruplex structures and other secondary structures are being exploited by mono and polynuclear metal complexes. Sequential binding and sensing of znii by bacillus. Over the last 30 years there has been increasing interest in utilizing kinetically inert transitionmetal complexes as noncovalent dna and rnabinding agents. Reactivity and structural studies of metal complexes bound. The effect of the copper complexes and the five different types of binding to dna observed in the simulations can be summarized comparing the structure from the clustering analysis with the crystal experimental structure of the free dna table 2. In continuance of our ongoing research on dna binding and cleavage happenings of transition metal complexes, in this paper we obtainable the synthesis, characterization and dna cleavage. Inhibitory activity against bacteria, spectrochimica acta part a. The mobility of the bands decreased as the concentration of the complex was increased, indicating that there was increase in the interaction between the metal.
Synthesis, structural elucidation and dna binding profile of. Dna binding, dna cleavage and bsa interaction of vanadium. The emission spectrum of 4 shows an intense fluorescence band centered at 420 nm which red shifts, slightly upon coordination of 4 to gold. Uv studies of the interaction of the compounds with dna reveal that these complexes can bind to ctdna and the binding constants have been calculated. Synthesis, characterisation and dna binding studies of metal. Synthesis, characterization, dna binding interaction. Synthesis, structural elucidation and dna binding profile. We have also investigated the interaction between the complexes and a sequence of duplex dna to gain some insight into the selectivity of the compounds for. Synthesis, characterization and biological evaluation of. Synthesis, characterization and antimicrobial screening mixed. Competitive dnabinding studies between metal complexes, cuphendionphenclcl 1, cuphendionebpyclcl 2, cudppt2h2opf6 3, nidppt2cl2 4, zndppt2cl2 5, and k3fecn6 6 where phen.
Our previous dnabinding studies with a series of amidoxime, ethanone oxime and aldoxime carboxylates as well as sulfonates of high, moderate or poor dna photocleaving ability showed no substantial differences on the dna affinities among the three kinds of oxime functionalities. All the metal complexes were screened for antibacterial activities. The path for metal complexes to a dna target ncbi nih. Introduction dna binding metal complexes have been extensively studied as dna structural probes, dna dependent electron probes and so on during the past decade. As already observed in the plots of rmsd versus time available in the supporting material, the. Synthesis chracterization and dna binding studies of novel. Synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy. Competitive dnabinding studies between metal complexes. Metallointercalators with different metal centers, oxidation states, coordination geometries, and overall sizes will exhibit varying depths of insertion. The dna binding studies of the cobalt complex containing the ligand smbtu is reported. Fluorescence and absorption studies of dnapdii complex.
Synthesis, characterization, antimicrobial, dna binding and cleavage studies of schiff base metal complexes by poomalai jayaseelan, selladurai prasad, subramanian vedanayaki and rangappan rajavel get pdf. The relative binding efficacy of the complexes to dna was much higher than the binding of ligands, especially the complex of cu l1 l2 cl 2 had the highest binding ability to dna. Synthesis, characterisation and dna binding studies of. An overview of the structures of proteindna complexes. Metal complexes that interact with dna have gained considerable interest as diagnostic agents and chemotherapeutic drugs 1,2. Proteins are gaining importance as nontraditional targets for metal complexes either by using the metal as a structural scaffold or by exploiting the reactivity of coordination. The antimicrobial and antifungal activity of the complexes have been studied against grampositive and gramnegative microorganisms and fungis and compared with the reference drug eno. Metallointercalators insert themselves between two intact base pairs without expelling or replacing the original nitrogenous bases. Synthesis, crystal structure, and dnabinding studies of a. Using fret melting assays, the binding affinity and selectivity of the five metal complexes against six different g4 dna structures as well as a.
Intercalation processes of copper complexes in dna nucleic. Here, this problem is explored in the context of the design and reactivity of different metallointercalators bound to dna. Synthesis, characterization, biological activity and dna binding. Studies on dna binding behaviour of biologically active. From the results, it can be concluded that both the complexes show intercalative binding mode with dna rather than groove binding. The metal complexes were characterized on the basis of various spectroscopic techniques like ir studies and elemental analysis while the geometry of complexes was octahedral which is confirmed by electronic spectra and thermogravim. Electronic absorption spectroscopy serves as the most common means to study the interactions between metal complexes and dna barton et al. Dna binding studies were carried out by viscosity method. There are two wellresolved bands at about 272, 278 nm for the. The molecular docking scores of 4, 5a, and 5b with bdna suggest a similar trend in behavior to that observed in the dnabinding study.
Intriguingly these studies reveal that dna damage resulting from ct is funneled into regulatory sites in mitochondria suggesting this may serve as a mechanism to protect the genomes of mitochondria. Synthesis, characterization, dna binding and cleavage. The dna cleavage studies of metal complexes presented more protruding activity in the attendance of h 2 o 2 associated to that in the absence of h 2 o 2. Transparency documentsupplementary data associated with this article can. For example, square planar complexes penetrate deeper into the dna. Among the transition metals, copper has also been extensively studied and proven as a promising candidate for. Binding studies of small molecules to dna are very important in the development of dna molecular probes and new therapeutic reagents. Truly, metal complexes bound to dna provide an interesting system to study structure, function and dynamics in a single package. Dna binding behaviors of these complexes with calf thymus dna ct dna have been investigated by absorption spectra, viscosity, and thermal denaturation methods. From small metal complexes in biological systems to metalloproteins. The interaction of vanadiummorin complex with calfthymus dna was monitored by uvvisible spectroscopy. Understanding how metal complexes interact with dna has become an active research area at the interface between chemistry, molecular biology and medicine. Our previous dna binding studies with a series of amidoxime, ethanone oxime and aldoxime carboxylates as well as sulfonates of high, moderate or poor dna photocleaving ability showed no substantial differences on the dna affinities among the three kinds of oxime functionalities.
Metal complexes, cefixime, dna binding studies, ligand. Synthesis, characterization, dna binding interaction studies. Reactivity and structural studies of metal complexes bound to dna. Design, syntheses and functional studies of dna binding metal complexes final seminar april 10, 1998 antitumor activity of many anticancer drugs is based on their interaction with dna five basic interaction types are observed. Synthesis, characterization, antimicrobial, dna binding and cleavage studies of schiff base metal complexes by poomalai jayaseelan, selladurai prasad, subramanian vedanayaki and rangappan rajavel get pdf 2 mb. The necessity for cellular regulation of dna led to the development of metallonucleases to catalyze and repair dna strand breaks.
Further dna was isolated from yeast cells and checked for purity, dna binding ability of both the complexes was studied by uv absorption titration technique. Threedimensional representations of various metal complex geometries. In concordance with the cytotoxicity studies, the dna binding studies. Evaluation of binding affinity with cancer causing. Even less defined structurally are other conformations of dna, some of which are illustrated schematically in figure 8.
Find, read and cite all the research you need on researchgate. The % fluorescence decrease is directly related to the extent of dna binding. Synthesis, dna binding, and molecular docking studies of. An overview is presented of the key role for heavymetal compounds in medicine with a focus on dna binding, and on pt compounds used in treatment of cancer.
Synthesis, spectroscopic, thermal properties, calf thymus. Structural data of dna binding and molecular docking studies. Jun 20, 2016 the method is based on the decrease of fluorescence derived from the displacement of gelred from dna by metal complexes. Dna binding behaviors of these complexes with calf thymus dna ctdna have been investigated by absorption spectra, viscosity, and thermal denaturation methods. Metallointercalation is a dna binding mode that has been extensively studied. Both schiff bases and other metal complexes were found to be inactive.
Metal ions and metal complexes have long been recognized as critically important components of nucleic acid chemistry, both in regulation of gene expression and as promising therapeutic agents. Read synthesis, dna binding, and antimicrobial studies of novel metal complexes containing a pyrazolone derivative schiff base, chemical papers on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Due to cationic character, threedimensional structural profiles, and propensity for performing hydrolysis, redox, or photoreactions of metal ions and complexes, have a natural ability for interacting with dna. Characterization of the dna binding specificity of.
Molecular and biomolecular spectroscopy on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. The absorption spectra of complex in aqueous solutions were compared in the absence and in the presence of ctdna are shown in the fig 1. On the basis of a structural analysis of 240 protein dna complexes contained in the protein data bank pdb, we have classified the dna binding proteins involved into eight different structuralfunctional groups, which are further classified into 54 structural families. Competitive dnabinding studies between metal complexes and. Design, syntheses and functional studies of dna binding metal. Dnabinding studies indicate that bszur is sequentially activated from an inactive dimer zur 2. Binding of metallointercalators to dna is reversible and depends on the properties of the intercalating molecule. Recent studies have shown that a variety of transition metal complexes have significant potential as probes for sequence and structurespecific dna binding 2. From the spectral studies an octahedral geometry was proposed for the copper complex. Pdf synthesis, characterization, dna binding properties. Structural data of dna binding and molecular docking studies of. Synthesis, structural elucidation and dna binding profile of znii bisbenzimidazole complexes mildred rodriguezcordero,1 noelani ciguela,1 ligia llovera,2 teresa gonzalez,2 alexander briceno,2 vanessa r. The results showed that the metal complexes are biologically active. Synthesis, characterization and antimicrobial screening.